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Xenograft Animal Service

Xenograft Animal Service: Immunocompromised NOD/SCID Mice

Xenografting is an established technique wherein tumor tissue from one species is transplanted into individuals of a different species. Xenografts of human tumor tissue into immunocompromised mice can provide a more accurate model of tumor growth and activity of administered drugs than in vitro studies. We offers in vivo xenograft services to conduct drug development for oncology, inflammation, diabetes, infectious diseases, obesity, immunology and pain research. Xenograft is a powerful research tool in oncology, playing a major role in development of new anti-cancer medicines.

Xenograft in vivo services are performed to measure the activity of compounds based on the rate of engrafted tumor growth. The xenotransplantation process involves administration of tumorigenic cell lines via subcutaneous injection. Alternative xenotransplantation approaches that can be performed include orthotopic, intramuscular, intravenous, intratracheal, or intraperitoneal administration.

We offers assistance in choosing the best model suitable for clients experimental goals. Animal handling and maintenance at our facility is IACUC-regulated and GLP-compliant. We provide detailed experiment procedures, health reports and experimental data. Additional experimental services include tissue collection, histology, RNA isolation and gene expression analysis.

BASIC STUDY DESIGN

8- to 12-week-old female BALB/c athymic (nude) mice (Nu/Nu) • 9-14 week old NOD/SCID immunocompromised mice (SCID)
Xenografting is accomplished by subcutaneous injection of tumor cell line cells (typically 1,000,000 cells per animal, single flank injection in 50% matrigel). Tumors are established in a group of mice (usually 5-10 animals per experimental group) and tumor growth is detectable within 4-7 days post implantation.

Clients select a cell line of interest (must be tumorigenic) and assign the number of experimental groups and the size of each group. The dosing of compounds is initiated when the mean tumor size reaches 50-100 mm3. Clients are expected to provide dose/group assignment instructions for each group.

Administration options include the dosing route (intratumoral, intramuscular, oral, intravenous, intratracheal, subcutaneous, or intraperitoneal), dosing frequency (for example, once or twice a day), and dosing duration. If the client elects to include the cyclophosphamide positive control group, cyclophosphamide (50 mg/kg) will be administered by intramuscular injection daily for the duration of the study.

We perform daily analysis with cage-side observations, and measure body weight and tumor volume every 2 days. Tumor volume (mm3) is calculated using the “(W x W x L) / 2″ formula, where W is tumor width and L is tumor length.

We offers multiple xenograft tumor models, including A549, DU-145, PC-3, U87MG, MDA-MB, PANC1, Caki-1, Caki-2, SKMEL, A431, LnCAP, FaDu, HeLa, SKOV-3, SW-480, and many other cell lines (see Table 1 below for most commonly used cell lines). Following acclimatization, mice are grouped according to body weight. Human cancer cell lines are prepared in matrigel and grafted subcutaneously into the flank area. Mice are checked daily for clinical signs and tumor appearance.

When tumors begin to appear, animals are grouped based on tumor volume. Mice are dosed once or twice a day for 28 days via the chosen route of administration (choice of oral, intratumoral, intraperitoneal, intratracheal, intramuscular, or subcutaneous). Other available services include collection of blood sera at termination, and preservation of tumors and other major tissues. Optional positive control group utilizing cyclophosphamide, cyclophosphamide (50 mg/kg) can be administered by intramuscular injection daily for the duration of the study.

Table 1. Some of the tumorigenic cell line maintained at Altogen Labs:

Tumor Type: Cell Lines
Brain SK-N-AS, U87 MG
Breast Cancer MCF-7, T-47D, MDA-MB-231
Colon HCT-15, COLO205, HT-29, SW-480
Gastric NCI-NC87, SNU-5
Hepatocellular / Liver Hep3B, SNU-398
Leukemia/Lymphoma K-562, MOLT-4, Raji, Ramos
Lung A549, NCI-H460, NCI-H69
Melanoma A2058, A375, SKMEL5
Multiple Myeloma RPMI 8226, H929, OPM-2
Ovarian OV-CAR3, SK-OV-3, IGR-0V1
Pancreas PANC-1, BxPc-3, Capan-1
Prostate 22Rv1, DU-145, PC-3
Renal / Kidney Caki-1, Caki-2, G-401
Thyroid 8505C, FTC-23

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