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Sequencing Services

Genomics Sequencing
Offering the expanding range of sequencing services available today, with applications in human, plant , animal, and microbial research, we can provide innovative, affordable, and reliable solutions for virtually any genomic challenge our customers and collaborators.

Our mission is to facilitate interdisciplinary genomics research by providing researchers access to cutting-edge technologies in the field of genomics.

Support all types of genomic research:

Our facilities/Partners are featured by next generation sequencing(NGS) and Sanger sequencing technologies, providing the high throughput sequencing capacity. We ascribe to the highest standards in genomics solutions for sample preparation, sequencing experiment design, and customized bioinformatics analysis, providing fast, accurate, and reliable sequencing services.

The sequencing platform is comprised of  HiSeq X, HiSeq 4000,  HiSeq 2500/HiSeq 2000,  MiSeq and  NextSeq500 sequencers. This platform can produce high quality data using different sequencing strategies, tailored to meet the diverse needs (library types, read length, throughput, turnaround time and cost).

Our genomics solutions include:

Next Generation Sequencing

DNA and RNA sequencing is the process of determining the nucleotide order of a given DNA fragment.  The nucleotide sequence encodes the necessary information that allows living things to survive and reproduce.  Determining the sequence is therefore useful in researching into how organisms live.  Our core facility provides many sequencing options to meet your individual needs, utilizing two different NGS platforms.

  • Whole transcriptome sequencing (RNA-Seq)
  • Small RNA sequencing (miRNA-Seq)
  • Whole exome sequencing
  • Targeted resequencing
  • Whole genome sequencing
  • Chromatin immunoprecipitation sequencing (ChIP-Seq)
  • Methylation sequencing (Methyl-Seq)
  • Copy number variation sequencing (CNV-Seq)

de novo Sequencing: Sequencers and data analysis solutions facilitate de novo sequencing and assembly of novel genomes.

Whole Genome Resequencing: Whole genome resequencing characterize the genetic diversity within the organism’s species or between closely related species, detect the full range of genetic variation such as SNPs, insertions, deletions, inversions, complex rearrangements, and copy number variation.

Exome and Target Region Sequencing: Current approaches become cost-prohibitive when working with large numbers of samples and regions, or demonstrate variable coverage. Exome sequencing and Targeted resequencing overcome these challenges.

Whole Genome Mapping You will be able to investigate microbial structure, function, diversity and genetics, without the need for amplification, PCR, cloning, paired-end libraries, pure isolates, or genomic specific reagents.

Human MHC-Seq: This new technology can type 26 HLA genes with 4-digit resolution and detect variants such as SNPs and InDels.

de novo Sequencing 
de novo sequencing, in which sequencing analysis is performed without the aid of a reference genome, is necessary when sequencing a novel species or novel DNA sequence. Combining the latest next generation sequencing technologies, you can obtain high quality results by sequencing different library types and subsequent state-of-the-art bioinformatics.

de novo Sequencing Features and Benefits:

Reliable genome assembly
Cost effective
High throughput data
Low background
Greater accuracy and broader dynamic range
de novo Sequencing Applications

Sequencing of unknown genomes or when no reference sequence is available. Samples with expected large structural variation e.g. cancer cells. Microbial sequencing – experimental strains, genomes with high plasticity.

de novo Sequencing Service includes:

The whole genome sequencing on human, animal, plant, microbial. The genome assembly, function annotations, comparative genomics and molecular evolution analysis.

Epigenomics Sequencing
Epigenetics involves the study of changes in the regulation of gene activity and expression that are not dependent on the DNA sequence. Epigenomics refers to a more global analysis of epigenetic changes across the entire genome. The epigenetic changes include DNA methylation and histone modification that have been developed to profile the epigenome using next generation sequencing platforms. We will arrange these in terms of library preparation, sequence platforms and analysis techniques.

We provides an array of epigenomics and bioinformatics research resources and services that include:

DNA methylation profiling
Protein-nucleic acid association (ChIP-Seq)
RNA-Seq
Small RNA-Seq
Genome Sequencing
Exome capture
Bisulfite Sequencing
Bioinformatics analysis
We will act as the main provider of NGS services on epigenomics in a variety of organisms ranging from animals (humans, mouse) to plants (maize, grapevine, Arabidopsis).

Exome & Target Region Sequencing 
Exome sequencing (also known as targeted exome capture) is an efficient strategy to selectively sequence the coding regions of the genome, which utilizes target enrichment strategy, as a cheaper but still effective alternative to whole genome sequencing.

Target region sequencing utilizes similar strategy which involves isolation of genomic regions of interest in a sample library, enabling cost effective and systematic detection of germline and somatic variants.

Exome and Target Region Sequencing Features and Benefits:

Targeted – focus on the regions of exome of interest.
Cost effective – much lower cost for narrowed region sequencing.
Precise – extremely high depth on the focused candidate sites or genes.
Exome and Target Region Sequencing Applications:

Resequencing of exomic regions
Polygenic disease studies
Genetic marker development
Large genomic loci resequencing
Resequencing of candidate gene sets
Metabolic pathway studies
Our Sequencing Service include:

Raw data as FASTQ files
Base calling and demultiplexing
Alignment to reference sequence
On-target and coverage statistics
Annotated SNPs and small InDels

FFPE Samples Sequencing 
Millions of archival FFPE samples provide an enormous and invaluable repository of information for genotyping studies. FFPE samples are preserved tissue samples that are generally associated with disease. These samples hold a wealth of data for biomarker discovery, drug development, and cancer research.

However, formidable challenging to get intact information from such samples, as severe degradation, or chemical modification could occur during sample preparation and store process. There are quite few usages of next generation sequencing (NGS) method in deciphering the FFPE nucleotide information. In order to unveil the information concealed in FFPE samples. To overcome this problem, the FFPE sample restoration solution provides a simple workflow to QC samples, repair damaged DNA, and achieve high-quality data.

Benefits:

Low input: As low as 200ng as for WGS, WES and RNA-Seq.
High throughput: Millions of reads product for downstream analysis.
Special analysis: Bioinformatics contents only for cancer tissue.On the GenSeqTM Technology, expression data from RNA purified from FFPE samples is highly correlated with data from RNA matched fresh samples. Furthermore, the capability of CD Genomics make our FFPE Samples Sequencing ideal for various types of clinical research studies and signature development, especially in cancer research.

Our core facility has various academic and commercial data mining software for genomic data analysis, including Partek Genomics Suite, NextGENe, IPA and Hoffman2 data analysis and storage server.

Turn-around Time

  • Sequencing
    • Library construction: 1-3 days
    • Sequencing: 3 days-3 weeks

To find out more details about this custom service, Please request a quote today.