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Screening / Development Services

ELISA and Cell-Based Assay Development 
ELISA and Cell-Based Assay Development (Cell Cycle, Viability) ELISA and Cell-Based Assays. We can develop a cell-based assay to quantify the effect of a client’s proprietary compound by quantitation of specific biomarkers, cytokines, or phosphoproteins in cell culture supernatant or whole-cell lysates. We uses a cell-based assay to analyze customer samples with a robust ELISA-based assay. The activity of the compound of interest is tested on a panel of 16 intracellular Ser/Thr kinases and 22 Tyr kinases, using phospho-specific antibodies by the determination of Substrate-specific phosphorylation activity induced by kinase activation, IC50 concentrations of Ser/Thr kinases and Tyr kinases.

Antibody Humanization 
We have extensive experience in antibody humanization. Humanization is important for reducing the immunogenicity of monoclonal antibodies derived from xenogeneic sources (commonly rodent) and for improving their activation of the human immune system. Since the development of the hybridoma technology, a large number of rodent monoclonal antibodies with specificity for antigens of therapeutic interest have been generated and characterized. Rodent antibodies are highly immunogenic in humans, which limits their clinical applications, especially when repeated administration is required. Importantly, they are rapidly removed from circulation and can cause systemic inflammatory effects as well. As a means of circumventing these problems, we have developed three antibody humanization strategies that can preserve the specificity and affinity of the antibody toward the antigen whereas significantly or completely eliminate the immunogenicity of the antibody in humans. The first approach is CDR grafting and the second approach is chain shuffling. These two methods are all based on phage display of humanized scFv variants and selection of high-affinity humanized binders through bio-panning. The third method, humanized IgG library screening, is somehow unique. We will make a library of humanized whole IgG to be displayed on the surface of mammalian cells and then high-affinity binders will be sorted by FACS.

Features
CDR Grafting & SDR Grafting
Chain Shuffling
Humanized IgG library screening

Membrane Protein Screening 
As a leading supplier for services in the biotechnology field, our services for membrane protein extraction, isolation and screening are open to any project, with a focus on the demanding class of membrane proteins. Our service includes a thorough discussion and counseling of your project, so that the methods used provide optimal conditions for your protein of interest.

Our service offering includes, but is not limited to

Membrane protein extraction from cell, cell lysate or cell culture supernatants.
Membrane protein isolation for cytoplasm membrane, mitochondrion membrane, chloroplast membrane, vacuole membrane or nucleus membrane.
Membrane protein screening by particles-ELISA.
Kinetic analysis of antibody binding by biosensor.
We use a kind of particle to provide an alternative to living cells, membrane preparations, and detergent-solubilized proteins by offering concentrated membrane proteins in their native conformation. Particles can be used in standard membrane protein assays and are compatible with a wide variety of detection platforms.

We provides technical support including experimental protocols and strict quality control using up to 16 metrics per batch.