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RNAi Services / miRNA / shRNA Services

We offer exceptional quality siRNAs to knock down your target genes. Custom siRNAs can be synthesized according to sequence information you provide, or you can take advantage of our complimentary siRNA design service*. Up to 30-mer siRNA including a choice of 32 different 3′ overhangs can be ordered with a variety of modification options for expanded specificity. siRNA is provided purified, annealed, lyophilized and ready-to-use. For even greater convenience, we offer Genome-wide Pre-designed siRNA – siRNAs pre-designed for human (18,084 genes), mouse (17,118 genes) and rat (9,392 genes) synthesized and ready-to-ship.

All custom siRNAs are synthesized in our state-of-the-art clean room facility and then purified free of charge. For higher purity, HPLC purification is available at an additional charge. Each siRNA is quality controlled by MALDI-TOF mass spectrometry to guarantee highest quality and analyzed by PAGE to confirm its duplex structure.

Features and Benefits

Guaranteed performance: Two of three custom siRNA will give 80% siRNA knockdown
Guaranteed Quality: Manufactured in a state-of-the-art clean room and QC’ed by MALDI-TOF and PAGE
Custom siRNA design service available: Turbo si-Designer software design is available free of charge
Competitive pricing: Overhang and annealing service provided free of charge.

shRNA Adenovirus Production, Full Service Pilot Scale

Recombinant Type 5 shRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
– One-stop service: from shRNA design to packaging.
– Unsurpassable high infection efficiency.
– Cost-effective.
– Special design request acceptable, FREE!

Large shRNA Ad.MAX™ shuttle vector collection offers tremendous flexibility for choosing a specific promoter and reporter.
Single-promoter Ad.MAX™ shuttle vector

Dual-promoter Ad.MAX™ shuttle vector

Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK

Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
CaMKII
cTNT
GFAP

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ

Service Description:
1. Synthesize and clone shRNA into adenovirus vector.
2. Viral production in Ad.MAX™ 293 cells: transfection and purification of recombinant adenovirus.
3. Generation of high titer recombinant adenoviral stock in ~500 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.

Required Materials: Sequence of gene of interest or shRNA.

Turnaround Time: 3 ~ 4 weeks.

Deliverables: 1.0 ml of high purity and high titer (1E+10 ~ 1E+11 PFU/ml*) adenovirus stock suitable for in vitro application.

To find out more details about this custom service, Please request a quote today.