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ELISA Antibody Testing

ELISA Antibody Testing

We develop or optimize the assays needed to test the efficacy of our clients’ products or therapies. Laboratory testing performed by our investigators have included immunologic assays (humoral and cellular immunity) and virologic assays. Humoral assays include assessment of antigen-specific serological antibody binding levels (ELISA, Western blot, RIPA, IFA, epitope mapping) and functional antibody (neutralizing antibody). Cellular assays to assess cell mediated immunity include cytokine ELISPOT, intracellular cytokine staining (ICC), memory ICC, cytometric bead array, lymphoproliferative response to specific viral proteins by thymidine incorporation or CFSE staining, expression of chemokines and CD8-T-cell antiviral activity. Virologic assays include: virus detection/quantification (isolation of virus from PBMC or tissues); determining levels of plasma/serum viral antigenemia; and performing molecular biological detection procedures for proviral DNA or viral RNA. We are working on developing new and novel assays with clients to more fully understand the entire immune response to their product or therapy.

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