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Protein Production Services

Protein Expression Services

Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged proteins for research to production of biopharmaceuticals for clinical use. Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. High level of protein expression also depends on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.

BACTERIAL PROTEIN EXPRESSION
Codon optimization for bacterial expression and gene synthesis of DNA insert
Generation of bacterial expression construct(s) in a selected or client-specified vector
Transformation into an appropriate host and preparation of glycerol stocks
Screening studies for the best-expressing protein variant
Optimization of growth conditions (host, induction, media, temperature, additives) to drive either soluble or inclusion bodies expression
Soluble vs. inclusion bodies expression assessment by SDS-PAGE/Coomassie or Western blot
Bacterial paste scale up production (read more Fermentation and Cell Culture Services)
Inclusion bodies wash and recovery
Recombinant Protein purification from soluble lysate fraction (read more Protein Purification Services)
Recombinant Protein purification from inclusion bodies by refolding (read more Protein Purification Services)
BACULOVIRUS PROTEIN EXPRESSION
Codon optimization for insect cell expression and gene synthesis of DNA insert
Subcloning of a DNA insert into a selected or client-specified baculovirus expression vector
Virus generation, amplification and cloning by limited dilution or plaque purification
Generation of high-titer virus stocks
SDS-PAGE, Western, ELISA or functional expression analysis of cell lysates and conditioned media for intracellular and secreted protein products
Secreted or intracellular expression in SF9, SF21 and High Five insect cells
Expression optimization studies: MOI, time course, growth media
Large-scale insect cell culture for conditioned media or cell pellet production
Recombinant protein purification from conditioned media or cell pellet (read more Protein Purification Services)
MAMMALIAN PROTEIN EXPRESSION
Codon optimization for mammalian cell expression and gene synthesis of DNA insert
Generation of mammalian protein expression construct(s) in a selected or client-specified vector
Endotoxin-free plasmid DNA preparation for transient or stable transfections
Small-scale trial to assess recombinant protein expression and/or to optimize transient transfection conditions
Various transient transfection reagent options
SDS-PAGE, Western, ELISA or functional expression analysis of conditioned media and/or cell lysates
Secreted or intracellular expression in CHO, HEK293, HEK293E or client-specified cells
Adherent or suspension cell growth options
Large-scale mammalian cell culture for conditioned media or cell pellet production
(read more Fermentation and Cell Culture Services)
Recombinant protein purification from conditioned media or cell pellet (read more Protein Purification Services)

cGMP-Manufacturing 

We provide process development and cGMP manufacturing services for biological active ingredients. We have ready access to state-of-the-art biopharmaceutical product development and manufacturing facilities and our staff scientists have demonstrable expertise in all aspects of biopharmaceutical manufacturing, from facility design and operation to the ability to provide best advice relating to product development. We also save our clients a great deal of time with bioprocess scale-up services. Our fermentation laboratories are equipped with state-of-the-art fermentors and bioreactors that enable us to express protein in large scale. Our customers can choose to enter into the protein expression pipeline at any stage depending on their needs.

We can offer you:

Construction of stable cell lines with certificated host cells and vectors.
GMP production of recombinant proteins and pharmaceutical antibodies.
Compliant with FDA Guidelines. Strict adherence to cGMP compliance and international intellectual properties.
Process development and scale up technologies for mammalian and microbial cell lines.
cGMP Support Services also include: Establishment and characterization of Master and Working Cell Banks, Stability Studies, analytical method development and qualification, regulatory support, and other GMP compliant requirements.
Featured Services:

cGMP Facility and Capacity
Theraputic Antibody & Protein Production
Bacterial Manufacturing
Manufacturing Cell Line Development
Process Development
Large-Scale Fermentation
Detailed Techniques Support
Our projects are as diverse as our customers, ranging from simple fill and finish to complex, aseptic manufacturing projects.

Risk-Free  Production Package

Risk Free Guarantees: protein quantity and purity are guaranteed. No Protein, No Charge!

Purity: choose from 85%/90%/95%, identified by SDS-PAGE and Western Blot (for tagged protein).
Quantity: choose from 3mg/5mg/10mg/15mg, identified by Bradford assay.
Timeline: 6-8 weeks

To find out more details about this custom service, Please request a quote today.