Request a Quote

Library Construction Services

Fosmid library construction service
We offers high quality fosmid library construction.

Fosmids are commonly used for preparing genomic libraries when a smaller insert size is desired. Fosmids are excellent candidates for closing gaps in a whole genome sequencing and physical mapping, metagenomic and expression screening and more due to their uniform coverage. The entire 40 kb insert can be shuttled into an expression vector more easily than a larger insert and the higher copy number per cell allows for easier manipulation and cheaper downstream applications.

Our Fosmid library construction service as follow:

Large Fosmid Insert length Available
Exceptional Yield & Small DNA Input Required
Easy production of Fosmid DNA.
Exceptional Success Rate.

Genomic library construction
Genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. then, using a host cell to carry the vectors allowing for easy amplification and retrieval of specific clones from the library for analysis.

We accumulated extensive experience in constructing large insert genomic libraries with different clone vectors.

Mutant library construction
In vitro molecular optimization is a very efficient means of generating mutant proteins with improved or novel properties, identifying regulatory sequences, and probing for structurally and functionally critical residues. Mutant libraries constructed using the in vitro molecular optimization method provide one useful approach to the systematic study of protein properties, regulation, and function.

Our mutant library service includes:

1. Site-directed mutagenesis libraries

Sunomix Biosciences combines its expertise in de novo gene synthesis and site-directed mutagenesis into an excellent site-directed mutagenesis library construction service. The site-directed mutagenesis library offers a great platform for protein function and active center studies. In these libraries, any given residue can be substituted with any of other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.

2. Scanning point mutation libraries

Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning by replacing each amino acid with all 20 amino acids simultaneously. This technique provides a detailed profile of each amino acid at the position. For each codon of interest, a small, site-saturated library is constructed. This library can be delivered as a pool or in a separated format for any substitution variant (19 in total). The application of our expertise in de novo gene synthesis to the field of sequential permutation scanning allows us to provide superb sequential permutation scanning library construction services.

3. Randomized and degenerated libraries

With our advanced degenerated oligonucleotide techniques, we can generate any form of randomization or degeneration of full-length gene in a synthetic DNA fragment. This permits controlled, highly precise randomization within oligonucleotides. Our in vitro library synthesis technology can introduce random substitutions on a controlled level with maximum flexibility. The mutation frequency can be set to any value between 1 and 20 mutations per kb. A peer group of 48, 96, or 192 individual transformants is sequence-verified.

BAC Library Construction Service 
BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. This technology was used in applications for genomic analysis that included large-scale physical mapping and genomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies.

This service should be attributed to the technical advantages of BAC cloning over YAC and cosmid cloning system summarized as follows:

Bigger insert size (up to 300 kb) than cosmid and other plasmid system.
Much more stable than yeast.
Bacterial clones and libraries grow much faster than YAC.
Easier in handling and gridding during DNA preparation.
Our BAC library construction service as follow:

Arrayed/non-arrayed BAC library
An arrayed or non-arrayed BAC library is the preferred choice for researchers wishing to screen using hybridization techniques. An arrayed library is also the only template that will do for whole genome sequencing or physical mapping.

Screened/Pooled BAC library

A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive

To find out more details about this custom service, Please request a quote today.